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Numerous professional suppliers of ligases use an arbitrary unit based on the ability of ligase to ligate cohesive ends. These units are frequently far more subjective than quantitative and absence precision.
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The action of E. coli DNA ligase may be Improved by DNA polymerase at the appropriate concentrations. Enhancement only is effective when the concentrations in the DNA polymerase 1 tend to be lower when compared to the DNA fragments being ligated.
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It is more steady and Energetic at Intense temperatures than standard DNA ligases. It is actually used in DNA amplification reactions to detect mutations in mammalian DNA.
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